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perturbs
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The answer PERTURBS has 13 possible clue(s) in existing crosswords.
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Definitions of perturbs in various dictionaries:
verb - disturb in mind or make uneasy or cause to be worried or alarmed
verb - disturb or interfere with the usual path of an electron or atom
verb - cause a celestial body to deviate from a theoretically regular orbital motion, especially as a result of interposed or extraordinary gravitational pull
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| Third-person singular simple present indicative form of perturb. |
| anxious or unsettled upset. |
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Perturb-seq (also known as CRISP-seq and CROP-seq) refers to a high-throughput method of performing single cell RNA sequencing (scRNA-seq) on pooled genetic perturbation screens. Perturb-seq combines multiplexed CRISPR mediated gene inactivations with single cell RNA sequencing to assess comprehensive gene expression phenotypes for each perturbation. Inferring a gene’s function by applying genetic perturbations to knock down or knock out a gene and studying the resulting phenotype is known as reverse genetics. Perturb-seq is a reverse genetics approach that allows for the investigation of phenotypes at the level of the transcriptome, to elucidate gene functions in many cells, in a massively parallel fashion. * The Perturb-seq protocol uses CRISPR technology to inactivate specific genes and DNA barcoding of each guide RNA to allow for all perturbations to be pooled together and later deconvoluted, with assignment of each phenotype to a specific guide RNA. Droplet-based microfluidics platforms (or other cell sorting and separating techniques) are used to isolate individual cells, and then scRNA-seq is performed to generate gene expression profiles for each cell. Upon completion of the protocol, bioinformatics analyses are conducted to associate each specific cell and perturbation with a transcriptomic profile that characterizes the consequences of inactivating each gene. * In the December 2016 issue of the Cell journal, two companion papers were published that each introduced and described this technique. A third paper describing a conceptually similar approach (termed CRISP-seq) was also published in the same issue. In October 2016, the CROP-seq method for single-cell CRISPR screening was presented in a preprint on bioRxiv and later published in the Nature Methods journal. While each paper shared the core principles of combining CRISPR mediated perturbation with scRNA-seq, their experimental, technological and analytical approaches differed in several aspects, to explore distinct biological questions, demonstrating the broad utility of this methodology. For example, the CRISPR-seq paper demonstrated the feasibility of in vivo studies using this technology, and the CROP-seq protocol facilitates large screens by providing a vector that makes the guide RNA itself readable (rather than relying on expressed barcodes), which allows for single-step guide RNA cloning. |